These differences can be used to distinguish the skin with dark circles from the normal skin. Meanwhile, the tissues of the skin with dark circles and normal skin demonstrated significant differences in MCI and MFI. The distribution of melanin granules in the pump-probe image and FeSO 4 staining was consistent. Subsequently, the melanin content index (MCI) and mean fluorescence intensity (MFI) were quantitatively analyzed by the pump-probe imaging. Furthermore, adjacent slices were respectively treated with hematoxylin–eosin (HE) and ferrous sulfate (FeSO 4) staining for cross-references.
Specimens obtained from 15 patients undergoing blepharoplasty were examined using pump-probe imaging. This study aimed to compare the distribution characteristics in melanin of lower eyelid skin tissues and to determine whether pump-probe imaging has potential for the classification of dark circles in vivo. an accumulation of plasma) in the under eye area.Pump-probe imaging was first used for quantitative analysis of melanin in dark circles’ skin to improve the ability to diagnose and treat dark circles on human skin. Regarding puffy eyes, two mechanisms may be accounted as responsible: the accumulation of fat and/or a local oedema (i.e. Each type requires a specific treatment in order to observe an improvement. There are several types of dark circles,1 and two are predominant: the dark circles due to an accumulation of melanin, and the dark circles due to an accumulation of red blood cells in the under eye area. Dark circles and puffy eyes however can be caused by multiple factors: they are therefore more difficult to treat, and few cosmetic compounds are actually capable of reducing these two unaesthetic conditions.ĭark circles, also known as periorbital hyperchromia, are characterised by bilateral homogenous macules and patches primarily involving the lower eyelid and the infraorbital region (under eye area). There are a lot of solutions for the treatment of crow’s feet wrinkles, from the topical application of a cosmetic grade anti-wrinkle to surgery and the injection of hyaluronic acid or botulinum toxin.
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